Name: FREDERICO PACHECO MILITÃO
Type: PhD thesis
Publication date: 09/03/2022
Advisor:
Name |
Role |
|---|---|
| Levi Pompermayer Machado | Co-advisor * |
| Valéria de Oliveira Fernandes | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| FERNANDA BRÊDA ALVES | External Examiner * |
| JEAN CARLOS VENCIONECK DUTRA | External Examiner * |
| Levi Pompermayer Machado | Co advisor * |
| Lucineide Maria Santana | External Alternate * |
| Maria do Carmo Pimentel Batitucci | Internal Examiner * |
| Stéfano Zorzal de Almeida | Internal Examiner * |
| Valéria de Oliveira Fernandes | Advisor * |
| Viviana Borges Corte | Internal Alternate * |
Summary: The main objective of the present study is to compile information about the biochemical composition of 10 microalgae isolated from fish farming ponds, necessary to select ideal species for biotechnological applications.The cultivation was of the batch type, without direct spraying of CO2, initial pH of 7 ± 0.05 and constant aeration of 3.5 L/min of air. The cultures were performed in quadruplicate in ASM1 (Cyanophyta) and BBM (Chlorophyta) medium, with an initial inoculum of 3.0 x105 Cel/mL-1, withthe exception of S. hirsutum, a species with slower growth, which was of 3250 Cel/mL-1, keeping the proportions.Statistical analyzes showed a significant difference in the kinetic parameters of growth between the species studied, with an increase in biomass. The highest concentrations of total soluble carbohydrates, at the end of the experiment, were found in the species Chlorococcumsp (12 mg/g DM), Kirchneriellasp (11.3 mg/g DM) and M. wesenbergii(11.3 mg/g DM). Chlorococcumsp also had the highest statistically significant concentrations of fructose (12.7 mg/g DM), sucrose (16.6 mg/g DM) and starch (934 mg/g DM). S. javanensis(357 mg/g DM), C. pulchra(343 mg/g DM), S. hirsutum (336 mg/g DM), Chlorococcum sp (307 mg/g DM), H. reticulata(306 mg/g DM), Kirchneriellasp (304 mg/g DM) and C. vulgaris(303 mg/g DM) did not differ statistically from each other in terms of the concentration of total soluble proteins.Microalgae isolated from fish-farmingtanks can be considered promising sources of biomass for raw material used to complement animal feed. The high starch content found also indicates a potential use of this biomass in the production of bioethanol.
